Effect of different irrigation protocols and calcium hydroxide dressing on the microhardness of root canal dentin

Abstract

Aim: This study aimed to evaluate the effect of different irrigation protocols and calcium hydroxide dressing on the microhardness of root canal dentin.

Methodology: Thirty human teeth were decoronated and root segments were obtained from pre-established cuts made below the cemento-enamel junction. After specimen’s preparation, the initial dentin microhardness (H0) was measured with a Vickers indenter, on the coronal surface, at 150 µm and 500 µm from the root canal lumen. Next, the specimens were randomly distributed into three groups (n=10), according to the different irrigation protocols: 1% NaOCl; 1% NaOCl + 17% EDTA; and 5% NaOCl. After irrigation, the hardness measurement was repeated (H1), in another quadrant. Then, Ca(OH)2 dressing was applied and left for 30 days, until it was removed and a new microhardness measurement (H2) was made, in a third quadrant. The data were statistically analyzed using the three-way analysis of variance (ANOVA) and Tukey tests, set at α=0.05.

Results: The factors analyzed (moment of VH, distance from the canal lumen and irrigation protocol) were statistically significant (p<0.05). There was a significant decrease of dentin microhardness after the irrigation protocols (H1<H0, p<0.05), however, with no significant difference after Ca(OH)2 dressing (H2~H1, p>0.05). The 5% NaOCl group shown the greatest difference between H0 and H1 measurements (p<0.05).

Conclusions: All irrigation protocols promoted significant decrease of the dentin microhardness. The Ca(OH)2 dressing for 30 days did not significantly affect the microhardness of the root canal dentin.

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Author Biographies

Ihan Vítor Cardoso, Federal University of Santa Catarina (UFSC)

Postgraduate Program in Dentistry of Federal University of Santa Catarina (UFSC)

Gabriela Rover, Federal University of Santa Catarina (UFSC)

Postgraduate Program in Dentistry of Federal University of Santa Catarina (UFSC)

Eduardo Antunes Bortoluzzi, Federal University of Santa Catarina (UFSC)

Associate Professor in Dentistry of Federal University of Santa Catarina (UFSC)

Lucas da Fonseca Roberti Garcia, Federal University of Santa Catarina (UFSC)

Associate Professor in Dentistry of Federal University of Santa Catarina (UFSC)

Cleonice Silveira Teixeira, Federal University of Santa Catarina (UFSC)

Associate Professor in Dentistry of Federal University of Santa Catarina (UFSC)

Published
2020-10-26
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Original Articles
Keywords:
calcium hydroxide, endodontics, hardness, root canal irrigants, sodium hyperclorite
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How to Cite
Pinto, L., Cardoso, I., Rover, G., Bortoluzzi, E., Garcia, L., & Teixeira, C. S. (2020). Effect of different irrigation protocols and calcium hydroxide dressing on the microhardness of root canal dentin. Giornale Italiano Di Endodonzia, 34(2). https://doi.org/10.32067/GIE.2020.34.02.08