Histomorphometric analysis of pulp submitted to the action of bromelain associated with irrigation solutions

  • Vinícius da Silva Caetano School of Dentistry, State University of Piaui, Parnaiba, PI, Brazil.
  • Even Herlany Pereira Alves Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
  • Luiz Felipe de Carvalho França Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
  • David di Lenardo Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
  • Victor Brito Dantas Martins Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
  • Juliana Gomes Galeno Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
  • Jefferson Soares de Oliveira Laboratory of Biology and Biochemistry Plants (BIOqPLANT), Federal University of Piaui, Parnaiba, PI, Brazil.
  • Daniel Fernando Pereira Vasconcelos | vasconcelos@ufpi.edu.br Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.

Abstract

Aim: The objective of this study was to evaluate the tissue disgregation capacity of pulp in different solutions associated or not with bromelain.

Methodology: Fifty bovine mandibular incisors were used, divided into five groups: C: Control, H: sodium hypochlorite 2.5%, B: bromelain, BL: bromelain + L-cysteine, BH: bromelain + sodium hypochlorite 2.5%. The pulp fragments were stored at -20 °C, washed with saline solution and moved to 25°C in eppendorf tubes with 1 ml of each substance. For the histomorphometric evaluation, the pulp fragments were prepared as histological routine, observed with optical microscopy and evaluated using ImageJ 1.49 software.

Results: The histomorphometric results showed that, in C: there was no alteration per tissue; H: there was a peripheral and central tissue disintegration; B: there was a protein and cellular degradation pattern, from the peripheral to the central area; BL: there was a central degradation. In BH, showed an effect enhanced by bromelain, when related to sodium hypochlorite, showing greater tissue degradation.

Conclusions: The association of bromelain with sodium hypochlorite potentiated the disintegration action of the pulp, opening possibilities for several clinical applications.

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Published
2019-06-11
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Original Articles
Keywords:
Bromelains, Endodontics, Root Canal Irrigants, Sodium Hypochlorite
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How to Cite
Caetano, V. da S., Pereira Alves, E. H., França, L. F. de C., di Lenardo, D., Martins, V. B. D., Galeno, J. G., de Oliveira, J. S., & Vasconcelos, D. F. P. (2019). Histomorphometric analysis of pulp submitted to the action of bromelain associated with irrigation solutions. Giornale Italiano Di Endodonzia, 33(1). https://doi.org/10.32067/gie.2019.33.01.03