Microbiological analysis of root canals of primary teeth with pulp necrosis caused by caries or trauma


Submitted: 10 November 2021
Accepted: 24 February 2022
Published: 2 May 2022
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Authors

  • Denise L. Sousa School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.
  • Rebecca B.R. Araújo School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.
  • Brenda P.F.A. Gomes Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil.
  • Juliana D. Bronzato Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil.
  • Thais M. Duque Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP and School of Dentistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Juliana O. Gondim School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.
  • José J. S. Moreira-Neto School of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, CE, Brazil.

Aim: This study aimed to investigate the microorganisms detected from root canals of primary teeth with pulp necrosis caused by dental caries or trauma.

Methodology: Microbial samples were taken from 44 cases in primary teeth with pulp necrosis either due to dental caries or trauma. DNA was extracted from the samples, which were analysed for the presence of fifteen endodontic pathogens by using PCR species-specific primers.

Results: The bacteria most detected in necrotic primary teeth due to caries (37/44) were P. micra (76.3%), P. nigrescens (76.3%), A. naeslundii (47.4%), and F. nucleatum (42.1%). On the other hand, A. naeslundii (83.3%), T. forsythia (83.3%), P. nigrescens (66.7%), and F. nucleatum (66.7%) were most frequently recovered from a root canal with pulp necrosis due to trauma (7/44). Significant associations were found between the presence of P. micra and the existence of caries (p=0.023) and sinus tract (p=0.044). The presence of T. forsythia was associated with the existence of trauma (p=0.035), and the presence of F. nucleatum was associated with pain on palpation (p=0.033).

Conclusions: The microbiota recovered from root canals of primary teeth with pulp necrosis caused by dental caries or trauma is similar, with the predominance of anaerobic microorganisms.


Supporting Agencies

Brazilian Agencies CAPES [financial code 001], CNPQ [308162/2014-5] and FAPESP [2015/23479-5].

L. Sousa, D., B.R. Araújo, R., Gomes, B. P., D. Bronzato, J., M. Duque, T., O. Gondim, J., & J. S. Moreira-Neto, J. (2022). Microbiological analysis of root canals of primary teeth with pulp necrosis caused by caries or trauma. Giornale Italiano Di Endodonzia, 36(1). https://doi.org/10.32067/GIE.2021.35.02.52

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