Assessing dentinal tubule penetration of an innovative bioactive glass-based root canal sealer through confocal laser scanning microscopy: an in vitro analysis
Accepted: 28 February 2024
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Aim: To assess and compare the dentinal tubule penetration of zinc oxide eugenol (ZOE)-based, resin-based, bioceramic, and novel bioactive glass-based root canal sealers using a confocal laser scanning microscope (CLSM).
Methods: A total of 48 single-rooted permanent teeth were categorized into four groups (n=12) and treated with gutta-percha along with ZOE sealer (Tubli-Seal EWT), resin-based sealer (AH Plus), bioceramic sealer (BioRoot RCS), and bioactive glass (Nishika Canal Sealer-BG). Cross-sections of the roots at 3 mm and 6 mm from the apex were examined under a CLSM to evaluate dentinal tubule penetration.
Results: Results indicated that bioceramic sealers exhibited the highest depth of dentinal tubule penetration at both levels, followed by bioactive glass and resin-based sealers. ZOE-based sealer demonstrated the least tubule penetration. Bioactive glass displayed the highest percentage of sealer penetration at 3 mm and 6 mm, with no statistically significant difference observed between bioceramic and bioactive glass groups regarding depth and percentage of dentinal tubule penetration at both levels.
Conclusions: Of particular significance is the bioactive glass group, demonstrating the most substantial sealer penetration percentage compared to the other groups at both examined depths.
Copyright (c) 2024 Sourabh Barbhai, Srinidhi SR, Rajesh Shetty, Poonam Joshi, Vini Mehta, Aida Meto
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