https://doi.org/10.32067/gie.2019.33.01.03

Histomorphometric analysis of pulp submitted to the action of bromelain associated with irrigation solutions

Vol. 33 No. 1 (2019)
Published: 11 June 2019
Bromelains, Endodontics, Root Canal Irrigants, Sodium Hypochlorite
Abstract Views: 713
pdf: 364
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Authors

Vinícius da Silva Caetano
School of Dentistry, State University of Piaui, Parnaiba, PI, Brazil.
Even Herlany Pereira Alves
Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
Luiz Felipe de Carvalho França
Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
David di Lenardo
Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
Victor Brito Dantas Martins
Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
Juliana Gomes Galeno
Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.
Jefferson Soares de Oliveira
Laboratory of Biology and Biochemistry Plants (BIOqPLANT), Federal University of Piaui, Parnaiba, PI, Brazil.
Daniel Fernando Pereira Vasconcelos
vasconcelos@ufpi.edu.br
Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piaui, Parnaiba, PI, Brazil.

Aim: The objective of this study was to evaluate the tissue disgregation capacity of pulp in different solutions associated or not with bromelain.

Methodology: Fifty bovine mandibular incisors were used, divided into five groups: C: Control, H: sodium hypochlorite 2.5%, B: bromelain, BL: bromelain + L-cysteine, BH: bromelain + sodium hypochlorite 2.5%. The pulp fragments were stored at -20 °C, washed with saline solution and moved to 25°C in eppendorf tubes with 1 ml of each substance. For the histomorphometric evaluation, the pulp fragments were prepared as histological routine, observed with optical microscopy and evaluated using ImageJ 1.49 software.

Results: The histomorphometric results showed that, in C: there was no alteration per tissue; H: there was a peripheral and central tissue disintegration; B: there was a protein and cellular degradation pattern, from the peripheral to the central area; BL: there was a central degradation. In BH, showed an effect enhanced by bromelain, when related to sodium hypochlorite, showing greater tissue degradation.

Conclusions: The association of bromelain with sodium hypochlorite potentiated the disintegration action of the pulp, opening possibilities for several clinical applications.

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How to Cite

Caetano, V. da S. ., Pereira Alves, E. H. ., França, L. F. de C. ., di Lenardo, D. ., Martins, V. B. D. ., Galeno, J. G., de Oliveira, J. S. ., & Vasconcelos, D. F. P. . (2019). Histomorphometric analysis of pulp submitted to the action of bromelain associated with irrigation solutions. Giornale Italiano Di Endodonzia, 33(1). https://doi.org/10.32067/gie.2019.33.01.03

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