In vitro cytotoxic effects of different intracanal medicaments on the co-culture of dental mesenchymal stem cells using the MTT assay

Abstract

Aim: This in vitro study aimed to assess the cytotoxic effects of different intracanal medicaments on the co-culture of dental mesenchymal stem cells.

Materials and Methods: The Periodontal Ligament Stem Cells (PDLSCs) and the stem cells of the apical papilla (SCAPs) were isolated from a human teeth. The cells were passaged and underwent mono-culture and co-culture. The cells were then exposed to different concentrations of calcium hydroxide/chlorhexidine, calcium hydroxide/distilled water, double antibiotic paste/ chlorhexidine, and double antibiotic paste/distilled water. The cytotoxicity and cell viability was evaluated and data were analyzed by independent t-test, ANOVA, and Tukey’s test (alpha=0.05).

Results: The viability of PDLSCs in co-culture was higher than that in mono-culture following exposure to different concentrations of medicaments. However, when distilled water was used as the vehicle for intracanal medicaments, the viability of SCAPs in mono-culture was higher than that in co-culture. In use of DAP/CHX, the viability of SCAP in co-culture was higher than that in mono-culture (P=0.000). This difference was not significant in exposure of SCAP to DAP/CHX (P>0.05).

Conclusions: Generally, the viability of PDLSCs in co-culture was higher than that in mono-culture, and distilled water was a better vehicle than CHX for intracanal medicaments.

 

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Published
2021-04-07
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Original Articles
Keywords:
antibiotics, cell viability, chlorhexidine, co-culture, stem cells
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How to Cite
Saberi, E. A., Gholami, F., Pirhaji, A., & Shahreki, E. (2021). In vitro cytotoxic effects of different intracanal medicaments on the co-culture of dental mesenchymal stem cells using the MTT assay. Giornale Italiano Di Endodonzia, 35(1). https://doi.org/10.32067/GIE.2021.35.01.13