Molecular Microbiological Analysis of ProTaper Next, XP-Endo Shaper and Reciproc Blue systems in severely curved canals

Abstract

Aim: This study evaluated the effectiveness of the mechanical reduction of intracanal bacteria produced by the endodontic systems Reciproc Blue (VDW GmbH), XP-Endo Shaper (FKG Dentaire) and ProTaper Next (Dentsply Sirona Endodontics) in severely curved canals by means of a molecular microbiological analysis.

Methodology: A total of 42 severely curved mesiobuccal canals of human permanent mandibular molars were selected and prepared. Then, canals were contaminated with Enterococcus faecalis strains (ATCC 29212) by incubation during 21 days at 37 ºC for formation of a mature biofilm. After that, contaminated specimens were randomly divided into 3 groups (n=14): ProTaper Next (G1), XP-Endo Shaper (G2) and Reciproc Blue (G3). Microbial samples were obtained before (S1) and after root canal preparation (S2). Analyses of intracanal E. faecalis reduction was performed using quantitative polymerase chain reaction (qPCR), and the differences between groups was analyzed by Kruskal-Wallis test. Significance level was set at P<0.05.

Results: All systems presented effective bacterial reduction, but still had bacterial growth. No significant difference between the evaluated file systems was demonstrated (P>0.05).

Conclusions: ProTaper Next, Reciproc Blue and XP-Endo Shaper presented similar mechanical reduction of intracanal bacteria. No file system was capable of rendering severely curved canals completely free from bacteria.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

References

1. Siqueira JF, Rôças IN. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97:85–94.
2. Machado MEL, Nabeshima CK, Leonardo MFP, Reis FAS, Britto MLB, Cai S. Influence of reciprocating single-file and rotary instrumentation on bacterial reduction on infected root canals. Int Endod J 2013;46:1083–7.
3. Haapasalo M, Shen Y. Evolution of nickel-titanium instruments: from past to future. Endod Top 2013;29:3–17.
4. Üreyen Kaya B, Erik CE, Sesli Çetin E, Köle M, Maden M. Mechanical reduction in intracanal Enterococcus faecalis when using three different single-file systems: an ex vivo comparative study. Int Endod J 2019;52:77–85.
5. Aksoy Ç, Keriş EY, Yaman SD, Ocak M, Geneci F, Çelik HH. Evaluation of XP-endo Shaper, Reciproc Blue, and ProTaper Universal NiTi Systems on Dentinal Microcrack Formation Using Micro–Computed Tomography. J Endod 2019;45:338–42.
6. Alves FRF, Rôças IN, Almeida BM, Neves MAS, Zoffoli J, Siqueira JF. Quantitative molecular and culture analyses of bacterial elimination in oval-shaped root canals by a single-file instrumentation technique. Int Endod J 2012;45:871–7.
7. Rôças IN, Lima KC, Siqueira JF. Reduction in bacterial counts in infected root canals after rotary or hand nickel-titanium instrumentation - a clinical study. Int Endod J 2013;46:681–7.
8. Neves MAS, Provenzano JC, Rôças IN, Siqueira JF. Clinical Antibacterial Effectiveness of Root Canal Preparation with Reciprocating Single-instrument or Continuously Rotating Multi-instrument Systems. J Endod 2016;42:25–9.
9. Amaral RR, Guimarães Oliveira AG, Braga T et al. Quantitative Assessment of the Efficacy of Two Different Single-file Systems in Reducing the Bacterial load in Oval- Shaped Canals: An In Vivo Study. J Endod 2020;46:1228–34.
10. De-Deus G, Belladonna FG, Zuolo AS et al. Effectiveness of Reciproc Blue in removing canal filling material and regaining apical patency. Int Endod J 2019;52:250–7.
11. Pérez AR, Ricucci D, Vieira GCS et al. Cleaning, Shaping, and Disinfecting Abilities of 2 Instrument Systems as Evaluated by a Correlative Micro–computed Tomographic and Histobacteriologic Approach. J Endod 2020;46:846–57.
12. Keles A, Uzunoglu Ozyurek E, Uyanik MO, Nagas E. Effect of Temperature of Sodium Hypochlorite on Cyclic Fatigue Resistance of Heat-treated Reciprocating Files. J Endod 2019;45:205–8.
13. Schneider SW. A comparison of canal preparations in straight and curved root canals. Oral Surgery, Oral Med Oral Pathol 1971;32:271–5.
14. Nabeshima CK, Caballero-Flores H, Cai S, Aranguren J, Britto MLB, De Lima Machado ME. Bacterial removal promoted by 2 single-file systems: Wave One and One Shape. J Endod 2014;40:1995–8.
15. Siqueira JF, Alves FRF, Versiani MA et al. Correlative bacteriologic and micro-computed tomographic analysis of mandibular molar mesial canals prepared by self-adjusting file, reciproc, and twisted file systems. J Endod 2013;39:1044–50.
16. Moshari A, Akhlaghi N, Rahimifard N, Darmiani S. Reduction of Enterococcus faecalis in curved root canals after various sizes and tapers of canal preparation. J Conserv Dent 2015;18:306–9.
17. Lacerda MFLS, Marceliano-Alves MF, Pérez AR et al. Cleaning and Shaping Oval Canals with 3 Instrumentation Systems: A Correlative Micro–computed Tomographic and Histologic Study. J Endod 2017;43:1878–84.
18. Matos Neto M, Santos SSF, Leão MVP, Habitante SM, Rodrigues JRDD, Jorge AOC. Effectiveness of three instrumentation systems to remove Enterococcus faecalis from root canals. Int Endod J 2012;45:435–8.
19. Ma J, Wang Z, Shen Y, Haapasalo M. A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy. J Endod 2011;37:1380–5.
20. Ferrer-Luque CM, Bejarano I, Ruiz-Linares M, Baca P. Reduction in Enteroccocus faecalis counts - A comparison between rotary and reciprocating systems. Int Endod J 2014;47:380–6.
21. Nakamura VC, Candeiro GT de M, Cai S, Gavini G. Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals. Braz Oral Res 2015;29:1–7.
22. Machado ME de L, Nabeshima CK, Caballero-Flores H et al. Instrument design may influence bacterial reduction during root canal preparation. Braz Dent J 2017;28:587–91.
23. Tahmasbi M, Jalali P, Nair MK, Barghan S, Nair UP. Prevalence of Middle Mesial Canals and Isthmi in the Mesial Root of Mandibular Molars: An In Vivo Cone-beam Computed Tomographic Study. J Endod 2017;43:1080–3.
Published
2021-05-06
Info
Issue
Section
Original Articles
Keywords:
bacterial reduction, molecular analysis, real-time polymerase chain reaction, root canal therapy, severely curved canals
Statistics
  • Abstract views: 140

  • PDF: 40
How to Cite
Nascimento, W. M., Montagner, M., Campos, D. L., Drumond, J. P., Maeda, W., Prado, M. C., de-Jesus-Soares, A., & Frozoni, M. (2021). Molecular Microbiological Analysis of ProTaper Next, XP-Endo Shaper and Reciproc Blue systems in severely curved canals. Giornale Italiano Di Endodonzia, 35(1). https://doi.org/10.32067/GIE.2021.35.01.15