@article{Nascimento_Montagner_Campos_Drumond_Maeda_Prado_de-Jesus-Soares_Frozoni_2021, title={Molecular microbiological analysis of ProTaper Next, XP-Endo Shaper and Reciproc Blue systems in severely curved canals}, volume={35}, url={https://www.giornaleitalianoendodonzia.it/gie/article/view/202}, DOI={10.32067/GIE.2021.35.01.15}, abstractNote={<p><strong>Aim: </strong>This study evaluated the effectiveness of the mechanical reduction of intracanal bacteria produced by the endodontic systems Reciproc Blue (VDW GmbH), XP-Endo Shaper (FKG Dentaire) and ProTaper Next (Dentsply Sirona Endodontics) in severely curved canals by means of a molecular microbiological analysis.</p> <p><strong>Methodology: </strong>A total of 42 severely curved mesiobuccal canals of human permanent mandibular molars were selected and prepared. Then, canals were contaminated with <em>Enterococcus faecalis </em>strains (ATCC 29212) by incubation during 21 days at 37 ºC for formation of a mature biofilm. After that, contaminated specimens were randomly divided into 3 groups (<em>n</em>=14): ProTaper Next (<em>G1</em>), XP-Endo Shaper (<em>G2</em>) and Reciproc Blue (<em>G3</em>). Microbial samples were obtained before (S1) and after root canal preparation (S2). Analyses of intracanal <em>E. faecalis</em> reduction was performed using quantitative polymerase chain reaction (qPCR), and the differences between groups was analyzed by Kruskal-Wallis test. Significance level was set at P<0.05.</p> <p><strong>Results: </strong>All systems presented effective bacterial reduction, but still had bacterial growth. No significant difference between the evaluated file systems was demonstrated (P>0.05).</p> <p><strong>Conclusions: </strong>ProTaper Next, Reciproc Blue and XP-Endo Shaper presented similar mechanical reduction of intracanal bacteria. No file system was capable of rendering severely curved canals completely free from bacteria.</p>}, number={1}, journal={Giornale Italiano di Endodonzia}, author={Nascimento, Wayne M. and Montagner, Mariana and Campos, Danilo L. and Drumond, João Paulo and Maeda, Walber and Prado, Marina C. and de-Jesus-Soares, Adriana and Frozoni, Marcos}, year={2021}, month={May} }