TY - JOUR AU - Diogo De-Carli, Alessandro AU - Luiz Sorgatto, Diego AU - Paim, Rennan AU - Colombo Vitali, Filipe AU - Aielo Bomfim, Rafael AU - de Carvalho Marion, Jefferson AU - Mageste Duque, Thais PY - 2021/03/03 Y2 - 2024/03/29 TI - Antimicrobial activity of tt-farnesol associated with an endodontic sealer against Enterococcus faecalis JF - Giornale Italiano di Endodonzia JA - G Ital Endodon VL - 35 IS - 1 SE - Original Articles DO - 10.32067/GIE.2021.35.01.12 UR - https://giornaleitalianoendodonzia.it/gie/article/view/185 SP - AB - <p><strong>Aim:</strong> This study aimed to evaluate <em>in vitro</em> the antibacterial activity of trans-trans farnesol (tt-farnesol) associated with Sealapex sealer against <em>Enterococcus faecalis</em>.</p><p><strong>Methodology: </strong>Initially, the minimum bactericidal concentration (MBC) of tt-farnesol was determined by microdilution technique. The sealer was associated with 350 µg/g tt-farnesol (G<sub>S+0.35f</sub>); 1750 µg/g tt-farnesol (G<sub>S+0.175f</sub>); or only Sealapex (G<sub>S</sub>). For antimicrobial activity test, <em>E faecalis </em>suspension was added in tubes containing the sealer samples and incubated for 24, 48, 72, 96, 120 and 144 h. After each time, two blinded and calibrated evaluators performed the CFU count. Data were analyzed statistically by <em>one way</em> ANOVA and Tukey <em>post-hoc</em> tests (significance level <em>P </em>&lt; 0.05).</p><p><strong>Results:</strong> It was observed difference in the CFU count between G<sub>S</sub>, G<sub>S+0.35f</sub> and G<sub>S+0.175 </sub>after 48 and 72 h, and between G<sub>S </sub>for the other groups in 96, 120 and 144 h (<em>P </em>&lt; 0.05). The CFU count was lower in G<sub>S+0.35f</sub> than in G<sub>S+0.175f</sub> after 48 and 72 h (<em>P </em>&lt; 0.05). In G<sub>S+0.35f, </sub>there was a decrease in CFU count after 48 h and in G<sub>S+0.175f </sub>after 72 h (<em>P </em>&lt; 0.01).</p><p><strong>Conclusions:</strong> The association of tt-farnesol with Sealapex decreased <em>E faecalis</em> growth <em>in vitro</em> after 48 h of incubation. The MBC of 0.35 and 0.175 mg/mL of tt-farnesol reduced the CFU count after 48 and 72 h, respectively.</p> ER -